Journal: OncoTargets and Therapy

Article Title:

Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/ott.s251926

Figure Lengend Snippet: Figure 3 mRNA expression of genes regulated by HPV 16E6 and NFX1-123 were significantly upregulated in cervical cancer. mRNA expression of 19 genes regulated by 16E6 and NFX1-123 were analyzed using TCGA Target TGEx in normal cervix (n = 10) compared to cervical primary tumors (n = 303). Significance was determined using two-tailed Mann–Whitney test. 14 of 19 genes were significantly upregulated in cervical tumors compared to normal tissue. LOR, CEBPD, LCE2B, FOXA2, and FBN2 were deregulated in cervical cancers, although they did not reach statistical significance. Note the Y-axis scales for individual gene expression datasets were adjusted to demonstrate samples with no detectable gene expression (0 value).

Article Snippet: Protein detection was performed with the following primary antibodies: RAET1G (Mouse RAET1G, 1:100 dilution, Santa Cruz Biotechnology, Cat No. sc-53134, Dallas, TX, USA); Notch1 (Rat Notch1 5B5, which detects both full-length Notch1 at 300kDa and its inactive NTM domain at 120kDa, 1:500 dilution, Cell Signaling Technology, Cat No.3447, Danvers, MA, USA); Transglutaminase 1 (Rabbit TGM1 1:500 dilution, Abcam, Cat No. ab27000, Cambridge, MA, USA); FOXA2 (Mouse HNF-3α/β (E-4) FOXA2, 1:100 dilution, Santa Cruz Biotechnology, Cat No. sc-377033, Dallas, TX, USA), and beta-actin (Mouse beta-actin (AC-15), 1:2000 dilution, Invitrogen, Cat No. AM4302, Waltham, MA USA).

Techniques: Expressing, Two Tailed Test, MANN-WHITNEY, Gene Expression

Journal: OncoTargets and Therapy

Article Title:

Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/ott.s251926

Figure Lengend Snippet: Figure 5 Protein expression of genes upregulated with overexpressed NFX1-123 and 16E6 in HPV positive cell lines. Western blot analysis of NFX1-123 and 16E6 regulated proteins (A) RAET1G and Notch1 (B) TGM1 and (C) FOXA2 in primary human foreskin keratinocytes (HFK), HPV 16 positive cervical cancer cell lines (SiHa and CaSki), and a HPV 18 positive cervical cancer cell line (HeLa). Beta- actin was used as a loading control. Fold expression of each protein of interest is shown for these cervical cancer cell lines relative to typical expression in HFKs.

Article Snippet: Protein detection was performed with the following primary antibodies: RAET1G (Mouse RAET1G, 1:100 dilution, Santa Cruz Biotechnology, Cat No. sc-53134, Dallas, TX, USA); Notch1 (Rat Notch1 5B5, which detects both full-length Notch1 at 300kDa and its inactive NTM domain at 120kDa, 1:500 dilution, Cell Signaling Technology, Cat No.3447, Danvers, MA, USA); Transglutaminase 1 (Rabbit TGM1 1:500 dilution, Abcam, Cat No. ab27000, Cambridge, MA, USA); FOXA2 (Mouse HNF-3α/β (E-4) FOXA2, 1:100 dilution, Santa Cruz Biotechnology, Cat No. sc-377033, Dallas, TX, USA), and beta-actin (Mouse beta-actin (AC-15), 1:2000 dilution, Invitrogen, Cat No. AM4302, Waltham, MA USA).

Techniques: Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: Heightened empathic responses led by imbalanced cortico-amygdala circuit in Shank3 autism mouse model

doi: 10.1101/2025.11.14.688290

Figure Lengend Snippet: A-C. Primer-KO was designed for identifying 65kb Shank3 DNA deletion between two loxp sites, which is located between exon 3 and 4, and after exon 22, respectively. Primer-ANK, Primer-PDZ and Primer-e21 were designed to evaluate Shank3 RNA knockdown efficacy in different loci of Shank3 gene ( A ). Genotyping of DNA from AAV-Cre-EGFP (Cre) or AAV-EGFP (GFP) viruses injected brain tissues showed that, Cre group presented amplification of a 500bp band but GFP group showed no PCR bands ( B ). RT-qPCR of RNA from virus injected brain tissues showed that Cre group showed reduction of Shank3 RNA expression in various regions of Shank3 gene( C ).

Article Snippet: Shank3 Δe4-22 and Shank3 e4-22 flox/flox mouse lines were generated in-house and have been deposited to the Jackson Lab (#039524, #039525).

Techniques: Knockdown, Injection, Amplification, Quantitative RT-PCR, Virus, RNA Expression

Journal: bioRxiv

Article Title: Heightened empathic responses led by imbalanced cortico-amygdala circuit in Shank3 autism mouse model

doi: 10.1101/2025.11.14.688290

Figure Lengend Snippet: A. Schematic drawing showed knockdown of Shank3 in Sh3 f/f mouse via injecting AAV-Cre-EGFP or AAV-EGFP viruses in ACC. Example images showed virus expression in ACC. Scale bar is 500 μm. B-E. Conditional deletion of Shank3 in ACC diminished social fear transfer. Compared to control group (ACC- Sh3 f/f +EGFP), cre group (ACC- Sh3 f/f +Cre) showed significant decreased freeze responses at SP but no change at RP. F. Schematic drawing showed knockdown of Shank3 in Sh3 f/f mouse via injecting AAV-Cre-EGFP or AAV-EGFP viruses in BLA. Example images showed virus expression in BLA. Scale bar is 500 μm. G-J. Conditional deletion of Shank3 in BLA enhanced social fear transfer process. Compared to control group (BLA- Sh3 f/f +EGFP), cre group (BLA- Sh3 f/f +Cre) showed significant increased freeze responses at both SP and RP.

Article Snippet: Shank3 Δe4-22 and Shank3 e4-22 flox/flox mouse lines were generated in-house and have been deposited to the Jackson Lab (#039524, #039525).

Techniques: Knockdown, Virus, Expressing, Control

Journal: Leukemia

Article Title: Sphingosine-1-phosphate Signalling drives an Angiogenic Transcriptional Programme in Diffuse Large B Cell Lymphoma

doi: 10.1038/s41375-019-0478-9

Figure Lengend Snippet: (A) Immunoblot of HUVEC pre-treated for 1h with 100nM of S1PR1 functional antagonists, Siponimod, Ozanimod and Ponesimod and stimulated with S1P or control (S1P vehicle) for 5min. S1P or control only treated cells received the equivalent dose of DMSO (drug solvent) to S1PR1 inhibitor treated cells (0.01µl DMSO per 1mL of media). β-tubulin was used as a loading control. The same protein lysates were used to detect pERK1/2 and tERK1/2 in separate blots. Data are representative of three separate donors. (B) Flow cytometry analysis of numbers of CD31-positive mouse cells/per mg tumour of SUDHL6 xenografts in NSG mice treated with Siponimod compared with drug vehicle (4 mice for each group). (C) Tumour volumes in days after injection with SUDHL6 cells in Siponimod-treated versus vehicle-treated animals. Arrows indicate treatment days. *denotes p<0.05 in Student’s t test.

Article Snippet: After 17 days (when tumour volume averaged 63mm 3 ) mice were randomised into two groups (each n=4) and treated orally with either vehicle (0.1% DMSO in 10% 2-hydroxypropyl-β-cyclodextrin; Cayman Chemical, MI, USA) or 6mg/kg Siponimod (Selleckchem.com, Munich, Germany) every 48h.

Techniques: Western Blot, Functional Assay, Control, Solvent, Flow Cytometry, Injection